Properties of Stylochiton Borumensis

The main objective of the present paper is to determine the total phenolic content, amino acid content, carbohydrate content and heavy metal determination by Folin-Ciocalteau reagent (F-C) method ,Ninhydrine method, anthrone method, inductively coupled plasma- atomic emission, respectively. And to verify the antimicrobial activity of the plant by disc diffusion method. The total phenolic content ranged from 340 to 500 mg/l gallic acid equivalent. The amino acids content the highest percentage was found in the ethanol extract (60.76%).Total carbohydrates were determined and the highest percentage was found in the ethanol extract (65.76%).Several metals including Pb, Cu, Cd and Zn were analyzed in S. borumensis roots by ICP-ES spectrophotometer and showed that it was in normal range compared to certified reference material the crude protein of S. borumensis roots was (7.15%). The ash content was a measure of the presence of inorganic compounds in drug. Ash content was (6.4%). The fiber content was (13.5%).The fats content was (2.1%).The antibacterial and antifungal activity of plant extracts with different concentrations (100, 50, 25, 12.5, 6.25mg ∕ ml) were investigated by disk diffusion assay, four extracts were tested against five micro-organisms; two strains of Gram-positive (Staphylococcus aureus, Bacillus subtilis), two strains of Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa) and one fungus (Candida albicans). The result showed that for all microorganisms the activity decreased by decreasing concentration of the extracts. The chloroform and ethanol 80% roots extracts gave the highest activity with inhibition zone (20mm and 17mm) respectively against Escherichia coli where reference antibiotic ciprofloxacin and erythromycin gave zone of (17mm and 12mm) respectively.

Keywords: Total phenolic content; antimicrobial activity; Stylochiton borumensis roots.